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hdac3 ncor2  (BPS Bioscience)


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    Structured Review

    BPS Bioscience hdac3 ncor2
    Hdac3 Ncor2, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 141 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hdac3+ncor2/HDAC3%2FNcoR2%2C+His-Tag%2C+GST-Tag+Recombinant/pmc13125535-601-29-40
    Average 94 stars, based on 141 article reviews
    hdac3 ncor2 - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Recombinant:

    Article Title: DCAF16‐Based Covalent Molecular Glues for Targeted Protein Degradation of Histone Deacetylases
    Article Snippet: .. Human recombinant HDAC1 (BPS Bioscience, Catalog# 50051), HDAC2 (BPS Bioscience, Catalog# 50052), HDAC3/NcoR2 (BPS Bioscience, Catalog# 50003), or HDAC6 (BPS Bioscience, Catalog# 50006) was used. ..

    Article Title: Sorbate induces lysine sorbylation through noncanonical activities of class I HDACs to regulate the expression of inflammation genes.
    Article Snippet: Sorbic acid (S1626- 100G), potassium sorbate (85520- 50G), Luminata Crescenodo horseradish peroxidase (HRP) substrates (WBLUR0500), Roche cOmplete protease inhibitor cocktail tablet (04693116001), N- 1- naphthylethylenediamine dihydrochloride (222488), nicotinamide (N3376), sulfanilamide (S9251), fetal bovine serum (F0926), LPS (L4391), Amicon filter (Ultra- 15), MISSION siRNA Universal Negative Control (sic001), and siRNAs targeting HDAC1 (SASI_Hs01_ 00079968) and HDAC2 (SASI_Hs01_00142115) were from MilliporeSigma (St. Louis, MO). siRNA targeting HDAC3 (hs.Ri.HDAC3.13.1) was from Integrated DNA Technologies (Coralville, IA). .. Recombinant HDAC1 (50051), HDAC2 (50002), and HDAC3/NCOR2 (50003) were from BPS Bioscience (San Diego, CA). .. Sorbic acid–13C2 (S676891) was from Toronto Research Chemicals (Toronto, Ontario, Canada).

    Article Title: Supporting Information Fluorinated Analogues of the HDAC Inhibitor Vorinostat (Zolinza): Validation of a Chiral Hybrid Bioisostere (BITE)
    Article Snippet: The in vitro inhibitory activities against human HDAC1, HDAC2, HDAC3/NcoR2 and HDAC6 were measured using a previously published protocol.1 OptiPlate-96 black microplates (Perkin Elmer) were used with an assay volume of 50 μL. .. 5.0 μL test compound or control, diluted in assay buffer (50 mM Tris-HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1.0 mM MgCl2, 0.1 mg/mL BSA), were incubated with 35 μL of the fluorogenic substrate ZMAL (Z-Lys(Ac)-AMC)2 (21.43 μM in assay buffer) and 10 μL of human recombinant HDAC1 (BPS Bioscience, Catalog# 50051), HDAC2 (BPS Bioscience, Catalog# 50052), HDAC3/NcoR2 (BPS Bioscience, Catalog# 50003) or HDAC6 (BPS Bioscience, Catalog# 50006) at 37 °C. .. After an incubation time of 90 min, 50 μL of 0.4 mg/mL trypsin in trypsin buffer (50 mM Tris-HCl, pH 8.0, 100 mM NaCl) were added, followed by further incubation at 37 °C for 30 min. Fluorescence was measured with an excitation wavelength of 355 nm and an emission wavelength of 460 nm using a Fluoroskan Ascent microplate reader (Thermo Scientific).

    Article Title: Multicomponent syntheses enable the discovery of novel quisinostat-derived chemotypes as histone deacetylase inhibitors.
    Article Snippet: OptiPlate-96 black microplates (PerkinElmer, USA) were used with an assay volume of 50 μL. .. 5.0 μL test compound or control, diluted in assay buffer (50 mM Tris-HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1.0 mM MgCl2, 0.1 mg/mL BSA), were incubated with 35 μL of the fluorogenic substrate ZMAL (ZLys (Ac)-AMC) [37] (21.43 μM in assay buffer) and 10 μL of human recombinant HDAC1 (BPS Bioscience, USA, Catalog# 50,051); HDAC2 (BPS Bioscience, USA, Catalog# 50,052); HDAC3/NcoR2 (BPS Bioscience, USA, Catalog# 50,003); or HDAC6 (BPS Bioscience, USA, Catalog# 50,006) at 37 ◦C. .. After an incubation time of 90 min, 50 μL of 0.4 mg/mL trypsin in trypsin buffer (50 mM Tris-HCl, pH 8.0, 100 mM NaCl) were added, followed by further incubation at 37 ◦C for 30 min. Fluorescence was measured with an excitation wavelength of 355 nm and an emission wavelength of 460 nm using a FLUOstar OPTIMA microplate reader (BMG Labtech).

    Article Title: Sorbate induces lysine sorbylation through noncanonical activities of class I HDACs to regulate the expression of inflammation genes
    Article Snippet: Sorbic acid (S1626-100G), potassium sorbate (85520-50G), Luminata Crescenodo horseradish peroxidase (HRP) substrates (WBLUR0500), Roche cOmplete protease inhibitor cocktail tablet (04693116001), N -1-naphthylethylenediamine dihydrochloride (222488), nicotinamide (N3376), sulfanilamide (S9251), fetal bovine serum (F0926), LPS (L4391), Amicon filter (Ultra-15), MISSION siRNA Universal Negative Control (sic001), and siRNAs targeting HDAC1 (SASI_Hs01_00079968) and HDAC2 (SASI_Hs01_00142115) were from MilliporeSigma (St. Louis, MO). siRNA targeting HDAC3 (hs.Ri.HDAC3.13.1) was from Integrated DNA Technologies (Coralville, IA). .. Recombinant HDAC1 (50051), HDAC2 (50002), and HDAC3/NCOR2 (50003) were from BPS Bioscience (San Diego, CA). .. Sorbic acid– 13 C 2 (S676891) was from Toronto Research Chemicals (Toronto, Ontario, Canada).

    Control:

    Article Title: Supporting Information Fluorinated Analogues of the HDAC Inhibitor Vorinostat (Zolinza): Validation of a Chiral Hybrid Bioisostere (BITE)
    Article Snippet: The in vitro inhibitory activities against human HDAC1, HDAC2, HDAC3/NcoR2 and HDAC6 were measured using a previously published protocol.1 OptiPlate-96 black microplates (Perkin Elmer) were used with an assay volume of 50 μL. .. 5.0 μL test compound or control, diluted in assay buffer (50 mM Tris-HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1.0 mM MgCl2, 0.1 mg/mL BSA), were incubated with 35 μL of the fluorogenic substrate ZMAL (Z-Lys(Ac)-AMC)2 (21.43 μM in assay buffer) and 10 μL of human recombinant HDAC1 (BPS Bioscience, Catalog# 50051), HDAC2 (BPS Bioscience, Catalog# 50052), HDAC3/NcoR2 (BPS Bioscience, Catalog# 50003) or HDAC6 (BPS Bioscience, Catalog# 50006) at 37 °C. .. After an incubation time of 90 min, 50 μL of 0.4 mg/mL trypsin in trypsin buffer (50 mM Tris-HCl, pH 8.0, 100 mM NaCl) were added, followed by further incubation at 37 °C for 30 min. Fluorescence was measured with an excitation wavelength of 355 nm and an emission wavelength of 460 nm using a Fluoroskan Ascent microplate reader (Thermo Scientific).

    Article Title: Multicomponent syntheses enable the discovery of novel quisinostat-derived chemotypes as histone deacetylase inhibitors.
    Article Snippet: OptiPlate-96 black microplates (PerkinElmer, USA) were used with an assay volume of 50 μL. .. 5.0 μL test compound or control, diluted in assay buffer (50 mM Tris-HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1.0 mM MgCl2, 0.1 mg/mL BSA), were incubated with 35 μL of the fluorogenic substrate ZMAL (ZLys (Ac)-AMC) [37] (21.43 μM in assay buffer) and 10 μL of human recombinant HDAC1 (BPS Bioscience, USA, Catalog# 50,051); HDAC2 (BPS Bioscience, USA, Catalog# 50,052); HDAC3/NcoR2 (BPS Bioscience, USA, Catalog# 50,003); or HDAC6 (BPS Bioscience, USA, Catalog# 50,006) at 37 ◦C. .. After an incubation time of 90 min, 50 μL of 0.4 mg/mL trypsin in trypsin buffer (50 mM Tris-HCl, pH 8.0, 100 mM NaCl) were added, followed by further incubation at 37 ◦C for 30 min. Fluorescence was measured with an excitation wavelength of 355 nm and an emission wavelength of 460 nm using a FLUOstar OPTIMA microplate reader (BMG Labtech).

    Incubation:

    Article Title: Supporting Information Fluorinated Analogues of the HDAC Inhibitor Vorinostat (Zolinza): Validation of a Chiral Hybrid Bioisostere (BITE)
    Article Snippet: The in vitro inhibitory activities against human HDAC1, HDAC2, HDAC3/NcoR2 and HDAC6 were measured using a previously published protocol.1 OptiPlate-96 black microplates (Perkin Elmer) were used with an assay volume of 50 μL. .. 5.0 μL test compound or control, diluted in assay buffer (50 mM Tris-HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1.0 mM MgCl2, 0.1 mg/mL BSA), were incubated with 35 μL of the fluorogenic substrate ZMAL (Z-Lys(Ac)-AMC)2 (21.43 μM in assay buffer) and 10 μL of human recombinant HDAC1 (BPS Bioscience, Catalog# 50051), HDAC2 (BPS Bioscience, Catalog# 50052), HDAC3/NcoR2 (BPS Bioscience, Catalog# 50003) or HDAC6 (BPS Bioscience, Catalog# 50006) at 37 °C. .. After an incubation time of 90 min, 50 μL of 0.4 mg/mL trypsin in trypsin buffer (50 mM Tris-HCl, pH 8.0, 100 mM NaCl) were added, followed by further incubation at 37 °C for 30 min. Fluorescence was measured with an excitation wavelength of 355 nm and an emission wavelength of 460 nm using a Fluoroskan Ascent microplate reader (Thermo Scientific).

    Article Title: Multicomponent syntheses enable the discovery of novel quisinostat-derived chemotypes as histone deacetylase inhibitors.
    Article Snippet: OptiPlate-96 black microplates (PerkinElmer, USA) were used with an assay volume of 50 μL. .. 5.0 μL test compound or control, diluted in assay buffer (50 mM Tris-HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1.0 mM MgCl2, 0.1 mg/mL BSA), were incubated with 35 μL of the fluorogenic substrate ZMAL (ZLys (Ac)-AMC) [37] (21.43 μM in assay buffer) and 10 μL of human recombinant HDAC1 (BPS Bioscience, USA, Catalog# 50,051); HDAC2 (BPS Bioscience, USA, Catalog# 50,052); HDAC3/NcoR2 (BPS Bioscience, USA, Catalog# 50,003); or HDAC6 (BPS Bioscience, USA, Catalog# 50,006) at 37 ◦C. .. After an incubation time of 90 min, 50 μL of 0.4 mg/mL trypsin in trypsin buffer (50 mM Tris-HCl, pH 8.0, 100 mM NaCl) were added, followed by further incubation at 37 ◦C for 30 min. Fluorescence was measured with an excitation wavelength of 355 nm and an emission wavelength of 460 nm using a FLUOstar OPTIMA microplate reader (BMG Labtech).



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